Blood Collection Tubes
Transcript Of Blood Collection Tubes
BLOOD COLLECTION TUBES
LIGHT BLUE TOP TUBE – 2.7 ml draw and 1.8 ml draw Liquid 3.2% sodium citrate anticoagulant
RED TOP TUBE – 4 ml draw Sprayed on clot activator
GOLD TOP TUBE (SST) – 3.5 ml draw Sprayed on clot activator with gel for serum separation
LIGHT GREEN TOP TUBE – 3 ml draw Lithium heparin anticoagulant with gel for plasma separation
PURPLE TOP TUBE – 2 ml draw or 4ml draw or 6ml draw Sprayed on K2EDTA Anticoagulant
The following tubes are available upon request through Catholic Medical Center’s Laboratory Customer Services:
NAVY BLUE TOP TUBE – 6 ml draw (trace element) No additive
NAVY BLUE TOP TUBE – 7 ml draw Na Heparin
NAVY BLUE TOP TUBE – 6 ml draw K2EDTA anticoagulant
GREEN TOP TUBE – 4 ml draw Sodium heparin anticoagulant
GRAY TOP TUBE – 6 ml draw Powder sodium fluoride / potassium oxalate anticoagulant
YELLOW TOP TUBE – 8.5 ml draw Liquid ACD solution A
YELLOW TOP TUBE – 6 ml draw Liquid ACD solution B
YELLOW TOP TUBE – 8.3 ml draw SPS (Sodium Polyanelthol Sulfonate)
After specimen collection, all tubes - with the exception of the Light Blue Top tube (Sodium Citrate) - are to be gently inverted 8-10 times. This will ensure the proper distribution of additives with the specimen. The Light Blue Top Sodium Citrate tube should be gently inverted only 3-4 times to avoid activation of platelets which could skew test results.
Gold and red top tubes should be allowed to clot for a minimum of 30 minutes and centrifuged within a maximum of 2 hours after collection. Serum for the red top tube must be removed as soon as possible from the cells to maintain the integrity of the specimen. Extra red top tubes that are collected in the emergency will only be viable for one hour on arrival in the lab.
Catholic Medical Center Laboratory 100 McGregor Street, Manchester, NH 03102
BD Vacutainer ® Venous Blood Collection
Tube Guide For the full array of BD Vacutainer® Blood Collection Tubes, visit www.bd.com/vacutainer.
Many are available in a variety of sizes and draw volumes (for pediatric applications). Refer to our website for full descriptions.
BD Vacutainer® Tubes
with BD Hemogard™ Closure
BD Vacutainer® Tubes with
Conventional Stopper
Gold
Red/ Gray
Additive
• Clot activator and gel for serum separation
Light Green
Green/ Gray
• Lithium heparin and gel for plasma separation
Inversions at Blood Collection*
5
8
Laboratory Use
For serum determinations in chemistry. May be used for routine blood donor screening and diagnostic testing of serum for infectious disease.** Tube inversions ensure mixing of clot activator with blood. Blood clotting time: 30 minutes.
For plasma determinations in chemistry. Tube inversions ensure mixing of anticoagulant (heparin) with blood to prevent clotting.
Your Lab’s Draw Volume/Remarks
Red Orange Royal Blue Green Gray Tan
Red
Gray/ Yellow
• Silicone coated (glass) • Clot activator, Silicone
coated (plastic)
• Thrombin
• Clot activator (plastic serum)
• K2EDTA (plastic)
Green Gray
• Sodium heparin • Lithium heparin
• Potassium oxalate/ sodium fluoride
• Sodium fluoride/Na2 EDTA • S odium fluoride
(serum tube)
• K2EDTA (plastic)
0
For serum determinations in chemistry.
5
May be used for routine blood donor
screening and diagnostic testing of serum
for infectious disease.** Tube inversions
ensure mixing of clot activator with blood.
Blood clotting time: 60 minutes.
8
For stat serum determinations in chemistry.
Tube inversions ensure mixing of clot activator
(thrombin) with blood to activate clotting.
8
For trace-element, toxicology, and
nutritional-chemistry determinations.
8
Special stopper formulation provides
low levels of trace elements
(see package insert). Tube inversions ensure
mixing of either clot activator or anticoagulant
(EDTA) with blood.
8
For plasma determinations in chemistry.
8
Tube inversions ensure mixing of anticoagulant
(heparin) with blood to prevent clotting.
8
For glucose determinations. Oxalate and
EDTA anticoagulants will give plasma
8
samples. Sodium fluoride is the
8 aenntsiugrleycporloytpiceramgeixnitn.gTuobfeadindvietirvseiownsith blood.
8
For lead determinations. This tube is certified
to contain less than .01 µg/mL(ppm) lead.
Tube inversions prevent clotting.
Lavender
White
Pink Light Blue Clear
• S odium polyanethol sulfonate (SPS)
8
SPS for blood culture specimen collections
in microbiology.
• A cid citrate dextrose
additives (ACD): Solution A -
ACD for use in blood bank studies, HLA
8
phenotyping, and DNA and paternity testing.
Yellow
22.0 g/L trisodium citrate,
8.0 g/L citric acid, 24.5 g/L
Tube inversions ensure mixing of anticoagulant
dextrose Solution B -
8
with blood to prevent clotting.
13.2 g/L trisodium citrate,
4.8 g/L citric acid, 14.7 g/L
dextrose
Lavender
• Liquid K3EDTA (glass) • Spray-coated K2EDTA
(plastic)
8
K2EDTA and K3EDTA for whole blood
8
hematology determinations. K2EDTA may be
used for routine immunohematology testing,
and blood donor screening.***
Tube inversions ensure mixing of anticoagulant
(EDTA) with blood to prevent clotting.
• K2EDTA with gel
8
For use in molecular diagnostic test methods
(such as, but not limited to, polymerase chain
reaction [PCR] and/or branched DNA [bDNA]
amplification techniques.) Tube inversions
ensure mixing of anticoagulant (EDTA) with
blood to prevent clotting.
• Spray-coated K2EDTA (plastic)
Pink
8
For whole blood hematology determinations.
May be used for routine immunohematology
testing and blood donor screening.***
Designed with special cross-match label for
patient information required by the AABB.
Tube inversions prevent clotting.
• Buffered sodium citrate
Light Blue
0.105 M (≈3.2%) glass 0.109 M (3.2%) plastic
• Citrate, theophylline,
adenosine, dipyridamole
(CTAD)
3-4
For coagulation determinations. CTAD for
selected platelet function assays and routine
coagulation determination. Tube inversions
3-4
ensure mixing of anticoagulant (citrate) to
prevent clotting.
Clear
New
Red/ Light Gray
• None (plastic)
0
For use as a discard tube or secondary
specimen tube.
Note: BD Vacutainer® Tubes for pediatric and partial draw applications can be found on our website.
BD Diagnostics Preanalytical Systems 1 Becton Drive Franklin Lakes, NJ 07417 USA
BD Global Technical Services: 1.800.631.0174 [email protected] BD Customer Service: 1.888.237.2762 www.bd.com/vacutainer
* Invert gently, do not shake ** The performance characteristics of these tubes have not been established for infectious disease testing in general; therefore, users must
validate the use of these tubes for their specific assay-instrument/reagent system combinations and specimen storage conditions. *** The performance characteristics of these tubes have not been established for immunohematology testing in general; therefore, users must
validate the use of these tubes for their specific assay-instrument/reagent system combinations and specimen storage conditions.
BD, BD Logo and all other trademarks are property of Becton, Dickinson and Company. © 2008 BD
Printed in USA
08/08 VS5229-9
How to Prepare a Quality Sample
Using BD Vacutainer® PST™ Tubes
Invert
8 -10
Times
Spin
10
Minutes
Ready for
Analysis
Centrifuge at 1100 - 1300g
x 8
Gently invert 8 -10 times immediately after collection to mix lithium heparin anticoagulant with blood.
Insufficient mixing may lead to microclot and fibrin strand formation.
Centrifuge at full speed
1100 – 1300g for 13 mm Plus Plastic tubes 1000 – 1300g for 16 mm Plus Plastic tubes
for 10 minutes in a swing-bucket unit or 15 minutes for a fixed-angle unit (balance tubes in centrifuge).
Gel barrier will form to separate plasma from red blood cells.
Use in laboratory for plasma determinations in chemistry.
BDPAS-PSTQualWC-7846.indd 1
3/6/06 12:27:10 PM
How to Prepare a Quality Sample
BD Vacutainer® Rapid Serum Tube
Invert
5-6 Times
Clot
5 Minutes
1500– 2000g
Spin
as low as
3*
Minutes
1500– 2000g 1500– 2000g
Swing-head Centrifuge
Swing-head Centrifuge Swing-head Centrifuge
x 5-6
Gently invert 5-6 times to mix clot activator with blood.
1500– 2000g
Swing-head Centrifuge
2000g
1500– 2000g
2000g 2000g
Swing-head Centrifuge
2000g
• Allow blood to clot for 4000g a minimum of 5 minutes in a vertical position.
• Observe a dense clot.
4000g
4000g
4000g
• Centr2i0f0u0gge at *4 000g for 3 minutes, 2000g for 4 minutes, or 1500–2000g for 10 minutes (balance tube in centrifuge), at 23-27°C.
4000g
• Barrier will form, separating serum specimen from clot.
• Transport spun tube to laboratory.
Ordering Information
Reference Number: 368774 Material: PET Tube Size (mm): 13 x 100 Draw Volume (mL): 5.0 Closure Type/Color: BD Hemogard™/Orange
Label Type: Paper V-Notch™
Additive: Thrombin‑based Clot Activator
Packaging Box/Case Quantities: 100/1000
For more information please contact your local BD Sales Consultant
BD Global Technical Services at 1.800.631.0174 BD Customer Service at 1.888.237.2762
BD, BD Logo and all other trademarks are property of Becton, Dickinson and Company. © 2010 BD VS8876
BD Diagnostics Preanalytical Systems 1 Becton Drive Franklin Lakes, NJ 07417 www.bd.com/vacutainer
PST BD Vacutainer® PST™ Tubes contain spray-coated lithium heparin
and a gel for plasma separation. They are used for plasma determinations in chemistry.
Reference #
367960 ✓
Tube Size (mm) 13x75
Draw Volume (mL) 3.0
Closure Type Color
Label type P
368056 NEW
13x75
3.0
P
367961
13x100
3.5
P
367962 ✓
13x100
4.5
P
367963
13x100
4.5
ST
367964 ✓
16x100
8.0
P
▲ BD CLINICAL ADVANTAGE:
✓= Best Practice Formulary
BD Vacutainer® PST™ Tubes eliminate the need to wait for a clot to form, making it an ideal tube for STAT testing, as well as for patients receiving anticoagulant therapy. The BD Vacutainer® PST™ Tube still provides the convenience of gel separation with the added advantage of improved turnaround time.
To learn more about the full product offering of BD Vacutainer® Specimen Collection Products or the educational materials and services offered by BD Diagnostics - Preanalytical Systems, please contact your local BD Sales Consultant. BD Global Technical Services: 1.800.631.0174 BD Customer Services: 1.888.237.2362 www.bd.com/vacutainer
BD, BD Logo, and all other trademarks are the property of Becton, Dickinson and Company. ©2006 BD. 03/06 VS7531
BDPAS-PSTQualWC-7846.indd 2
BD Diagnostics Preanalytical Systems 1 Becton Drive Franklin Lakes, NJ 07417 www.bd.com/vacutainer
3/6/06 12:27:13 PM
BD Vacutainer® Plus Plastic Citrate Tube
BD Vacutainer® Plus Plastic Citrate Tube Draw Volume Guide
Ref. # 363083
Ref. # 363080
Sufficient volume achieved if blood drawn falls above minimum fill indicator. For blood transfer, do not fill above illustrated dashed maximum line.
Maximum Fill*
Minimum Fill Indicator
Note: The quantity of blood drawn into evacuated tubes varies with altitude, ambient temperature, barometric pressure, tube age, venous pressure and filling technique.
2.7 mL Draw Tube
13 mm x 75 mm Full Draw
*According to CLSI guideline, Dec. 2003, Doc. H1-A5, Vol. 23, No. 33.
Now with Minimum Fill
Indicator
Represents minimum volume of blood required for
appropriate analysis
1.8 mL Draw Tube
13 mm x 75 mm Full Draw
BD Vacutainer® Plus Plastic Citrate Tube
Minimizing Preanalytical Variables for Coagulation Tests
• Assemble needle in holder; always fully seat and hold a citrate tube on the back end of the needle while filling.
• Allow the tube to fill until the vacuum is exhausted and blood flow ceases.
• Tubes should fill between ±10% of the stated draw volume of the tube (CLSI guideline, Dec. 2003, Doc. H1-A5, Vol. 23, No. 33).
• Minimum fill indicator represents the minimum volume of blood required for appropriate analysis.
• A discard tube (without additives) must be used if only a citrate tube is to be drawn using a winged blood collection set. It is important to remove the air from the blood collection set to ensure the proper blood volume is obtained in the tube.
• Do not fill tubes from other tubes or combine two partially filled citrate tubes.
• If the specimen is drawn with a syringe, do not fill the BD Vacutainer® Citrate Tube beyond the level as illustrated on the reverse side of this guide. Allow the tube to draw the blood from the syringe using a BD Vacutainer® Blood Transfer Device if available. Do not force blood into tube.
• Immediately after draw, gently invert tube 3 to 4 times. Do not shake.
Cat# 363083 363080
Size 13 x 75 mm 13 x 75 mm
Draw 2.7 mL 1.8 mL
Citrate 3.2% (0.109M) 3.2% (0.109M)
BD Global Technical Services: 1.800.631.0174 www.bd.com/vacutainer
BD, BD Logo and BD Vacutainer® are trademarks of Becton, Dickinson and Company. © 2007 BD Patents Pending 10/07 VS5944-3
BD Diagnostics Preanalytical Systems 1 Becton Drive Franklin Lakes, NJ 07417
LIGHT BLUE TOP TUBE – 2.7 ml draw and 1.8 ml draw Liquid 3.2% sodium citrate anticoagulant
RED TOP TUBE – 4 ml draw Sprayed on clot activator
GOLD TOP TUBE (SST) – 3.5 ml draw Sprayed on clot activator with gel for serum separation
LIGHT GREEN TOP TUBE – 3 ml draw Lithium heparin anticoagulant with gel for plasma separation
PURPLE TOP TUBE – 2 ml draw or 4ml draw or 6ml draw Sprayed on K2EDTA Anticoagulant
The following tubes are available upon request through Catholic Medical Center’s Laboratory Customer Services:
NAVY BLUE TOP TUBE – 6 ml draw (trace element) No additive
NAVY BLUE TOP TUBE – 7 ml draw Na Heparin
NAVY BLUE TOP TUBE – 6 ml draw K2EDTA anticoagulant
GREEN TOP TUBE – 4 ml draw Sodium heparin anticoagulant
GRAY TOP TUBE – 6 ml draw Powder sodium fluoride / potassium oxalate anticoagulant
YELLOW TOP TUBE – 8.5 ml draw Liquid ACD solution A
YELLOW TOP TUBE – 6 ml draw Liquid ACD solution B
YELLOW TOP TUBE – 8.3 ml draw SPS (Sodium Polyanelthol Sulfonate)
After specimen collection, all tubes - with the exception of the Light Blue Top tube (Sodium Citrate) - are to be gently inverted 8-10 times. This will ensure the proper distribution of additives with the specimen. The Light Blue Top Sodium Citrate tube should be gently inverted only 3-4 times to avoid activation of platelets which could skew test results.
Gold and red top tubes should be allowed to clot for a minimum of 30 minutes and centrifuged within a maximum of 2 hours after collection. Serum for the red top tube must be removed as soon as possible from the cells to maintain the integrity of the specimen. Extra red top tubes that are collected in the emergency will only be viable for one hour on arrival in the lab.
Catholic Medical Center Laboratory 100 McGregor Street, Manchester, NH 03102
BD Vacutainer ® Venous Blood Collection
Tube Guide For the full array of BD Vacutainer® Blood Collection Tubes, visit www.bd.com/vacutainer.
Many are available in a variety of sizes and draw volumes (for pediatric applications). Refer to our website for full descriptions.
BD Vacutainer® Tubes
with BD Hemogard™ Closure
BD Vacutainer® Tubes with
Conventional Stopper
Gold
Red/ Gray
Additive
• Clot activator and gel for serum separation
Light Green
Green/ Gray
• Lithium heparin and gel for plasma separation
Inversions at Blood Collection*
5
8
Laboratory Use
For serum determinations in chemistry. May be used for routine blood donor screening and diagnostic testing of serum for infectious disease.** Tube inversions ensure mixing of clot activator with blood. Blood clotting time: 30 minutes.
For plasma determinations in chemistry. Tube inversions ensure mixing of anticoagulant (heparin) with blood to prevent clotting.
Your Lab’s Draw Volume/Remarks
Red Orange Royal Blue Green Gray Tan
Red
Gray/ Yellow
• Silicone coated (glass) • Clot activator, Silicone
coated (plastic)
• Thrombin
• Clot activator (plastic serum)
• K2EDTA (plastic)
Green Gray
• Sodium heparin • Lithium heparin
• Potassium oxalate/ sodium fluoride
• Sodium fluoride/Na2 EDTA • S odium fluoride
(serum tube)
• K2EDTA (plastic)
0
For serum determinations in chemistry.
5
May be used for routine blood donor
screening and diagnostic testing of serum
for infectious disease.** Tube inversions
ensure mixing of clot activator with blood.
Blood clotting time: 60 minutes.
8
For stat serum determinations in chemistry.
Tube inversions ensure mixing of clot activator
(thrombin) with blood to activate clotting.
8
For trace-element, toxicology, and
nutritional-chemistry determinations.
8
Special stopper formulation provides
low levels of trace elements
(see package insert). Tube inversions ensure
mixing of either clot activator or anticoagulant
(EDTA) with blood.
8
For plasma determinations in chemistry.
8
Tube inversions ensure mixing of anticoagulant
(heparin) with blood to prevent clotting.
8
For glucose determinations. Oxalate and
EDTA anticoagulants will give plasma
8
samples. Sodium fluoride is the
8 aenntsiugrleycporloytpiceramgeixnitn.gTuobfeadindvietirvseiownsith blood.
8
For lead determinations. This tube is certified
to contain less than .01 µg/mL(ppm) lead.
Tube inversions prevent clotting.
Lavender
White
Pink Light Blue Clear
• S odium polyanethol sulfonate (SPS)
8
SPS for blood culture specimen collections
in microbiology.
• A cid citrate dextrose
additives (ACD): Solution A -
ACD for use in blood bank studies, HLA
8
phenotyping, and DNA and paternity testing.
Yellow
22.0 g/L trisodium citrate,
8.0 g/L citric acid, 24.5 g/L
Tube inversions ensure mixing of anticoagulant
dextrose Solution B -
8
with blood to prevent clotting.
13.2 g/L trisodium citrate,
4.8 g/L citric acid, 14.7 g/L
dextrose
Lavender
• Liquid K3EDTA (glass) • Spray-coated K2EDTA
(plastic)
8
K2EDTA and K3EDTA for whole blood
8
hematology determinations. K2EDTA may be
used for routine immunohematology testing,
and blood donor screening.***
Tube inversions ensure mixing of anticoagulant
(EDTA) with blood to prevent clotting.
• K2EDTA with gel
8
For use in molecular diagnostic test methods
(such as, but not limited to, polymerase chain
reaction [PCR] and/or branched DNA [bDNA]
amplification techniques.) Tube inversions
ensure mixing of anticoagulant (EDTA) with
blood to prevent clotting.
• Spray-coated K2EDTA (plastic)
Pink
8
For whole blood hematology determinations.
May be used for routine immunohematology
testing and blood donor screening.***
Designed with special cross-match label for
patient information required by the AABB.
Tube inversions prevent clotting.
• Buffered sodium citrate
Light Blue
0.105 M (≈3.2%) glass 0.109 M (3.2%) plastic
• Citrate, theophylline,
adenosine, dipyridamole
(CTAD)
3-4
For coagulation determinations. CTAD for
selected platelet function assays and routine
coagulation determination. Tube inversions
3-4
ensure mixing of anticoagulant (citrate) to
prevent clotting.
Clear
New
Red/ Light Gray
• None (plastic)
0
For use as a discard tube or secondary
specimen tube.
Note: BD Vacutainer® Tubes for pediatric and partial draw applications can be found on our website.
BD Diagnostics Preanalytical Systems 1 Becton Drive Franklin Lakes, NJ 07417 USA
BD Global Technical Services: 1.800.631.0174 [email protected] BD Customer Service: 1.888.237.2762 www.bd.com/vacutainer
* Invert gently, do not shake ** The performance characteristics of these tubes have not been established for infectious disease testing in general; therefore, users must
validate the use of these tubes for their specific assay-instrument/reagent system combinations and specimen storage conditions. *** The performance characteristics of these tubes have not been established for immunohematology testing in general; therefore, users must
validate the use of these tubes for their specific assay-instrument/reagent system combinations and specimen storage conditions.
BD, BD Logo and all other trademarks are property of Becton, Dickinson and Company. © 2008 BD
Printed in USA
08/08 VS5229-9
How to Prepare a Quality Sample
Using BD Vacutainer® PST™ Tubes
Invert
8 -10
Times
Spin
10
Minutes
Ready for
Analysis
Centrifuge at 1100 - 1300g
x 8
Gently invert 8 -10 times immediately after collection to mix lithium heparin anticoagulant with blood.
Insufficient mixing may lead to microclot and fibrin strand formation.
Centrifuge at full speed
1100 – 1300g for 13 mm Plus Plastic tubes 1000 – 1300g for 16 mm Plus Plastic tubes
for 10 minutes in a swing-bucket unit or 15 minutes for a fixed-angle unit (balance tubes in centrifuge).
Gel barrier will form to separate plasma from red blood cells.
Use in laboratory for plasma determinations in chemistry.
BDPAS-PSTQualWC-7846.indd 1
3/6/06 12:27:10 PM
How to Prepare a Quality Sample
BD Vacutainer® Rapid Serum Tube
Invert
5-6 Times
Clot
5 Minutes
1500– 2000g
Spin
as low as
3*
Minutes
1500– 2000g 1500– 2000g
Swing-head Centrifuge
Swing-head Centrifuge Swing-head Centrifuge
x 5-6
Gently invert 5-6 times to mix clot activator with blood.
1500– 2000g
Swing-head Centrifuge
2000g
1500– 2000g
2000g 2000g
Swing-head Centrifuge
2000g
• Allow blood to clot for 4000g a minimum of 5 minutes in a vertical position.
• Observe a dense clot.
4000g
4000g
4000g
• Centr2i0f0u0gge at *4 000g for 3 minutes, 2000g for 4 minutes, or 1500–2000g for 10 minutes (balance tube in centrifuge), at 23-27°C.
4000g
• Barrier will form, separating serum specimen from clot.
• Transport spun tube to laboratory.
Ordering Information
Reference Number: 368774 Material: PET Tube Size (mm): 13 x 100 Draw Volume (mL): 5.0 Closure Type/Color: BD Hemogard™/Orange
Label Type: Paper V-Notch™
Additive: Thrombin‑based Clot Activator
Packaging Box/Case Quantities: 100/1000
For more information please contact your local BD Sales Consultant
BD Global Technical Services at 1.800.631.0174 BD Customer Service at 1.888.237.2762
BD, BD Logo and all other trademarks are property of Becton, Dickinson and Company. © 2010 BD VS8876
BD Diagnostics Preanalytical Systems 1 Becton Drive Franklin Lakes, NJ 07417 www.bd.com/vacutainer
PST BD Vacutainer® PST™ Tubes contain spray-coated lithium heparin
and a gel for plasma separation. They are used for plasma determinations in chemistry.
Reference #
367960 ✓
Tube Size (mm) 13x75
Draw Volume (mL) 3.0
Closure Type Color
Label type P
368056 NEW
13x75
3.0
P
367961
13x100
3.5
P
367962 ✓
13x100
4.5
P
367963
13x100
4.5
ST
367964 ✓
16x100
8.0
P
▲ BD CLINICAL ADVANTAGE:
✓= Best Practice Formulary
BD Vacutainer® PST™ Tubes eliminate the need to wait for a clot to form, making it an ideal tube for STAT testing, as well as for patients receiving anticoagulant therapy. The BD Vacutainer® PST™ Tube still provides the convenience of gel separation with the added advantage of improved turnaround time.
To learn more about the full product offering of BD Vacutainer® Specimen Collection Products or the educational materials and services offered by BD Diagnostics - Preanalytical Systems, please contact your local BD Sales Consultant. BD Global Technical Services: 1.800.631.0174 BD Customer Services: 1.888.237.2362 www.bd.com/vacutainer
BD, BD Logo, and all other trademarks are the property of Becton, Dickinson and Company. ©2006 BD. 03/06 VS7531
BDPAS-PSTQualWC-7846.indd 2
BD Diagnostics Preanalytical Systems 1 Becton Drive Franklin Lakes, NJ 07417 www.bd.com/vacutainer
3/6/06 12:27:13 PM
BD Vacutainer® Plus Plastic Citrate Tube
BD Vacutainer® Plus Plastic Citrate Tube Draw Volume Guide
Ref. # 363083
Ref. # 363080
Sufficient volume achieved if blood drawn falls above minimum fill indicator. For blood transfer, do not fill above illustrated dashed maximum line.
Maximum Fill*
Minimum Fill Indicator
Note: The quantity of blood drawn into evacuated tubes varies with altitude, ambient temperature, barometric pressure, tube age, venous pressure and filling technique.
2.7 mL Draw Tube
13 mm x 75 mm Full Draw
*According to CLSI guideline, Dec. 2003, Doc. H1-A5, Vol. 23, No. 33.
Now with Minimum Fill
Indicator
Represents minimum volume of blood required for
appropriate analysis
1.8 mL Draw Tube
13 mm x 75 mm Full Draw
BD Vacutainer® Plus Plastic Citrate Tube
Minimizing Preanalytical Variables for Coagulation Tests
• Assemble needle in holder; always fully seat and hold a citrate tube on the back end of the needle while filling.
• Allow the tube to fill until the vacuum is exhausted and blood flow ceases.
• Tubes should fill between ±10% of the stated draw volume of the tube (CLSI guideline, Dec. 2003, Doc. H1-A5, Vol. 23, No. 33).
• Minimum fill indicator represents the minimum volume of blood required for appropriate analysis.
• A discard tube (without additives) must be used if only a citrate tube is to be drawn using a winged blood collection set. It is important to remove the air from the blood collection set to ensure the proper blood volume is obtained in the tube.
• Do not fill tubes from other tubes or combine two partially filled citrate tubes.
• If the specimen is drawn with a syringe, do not fill the BD Vacutainer® Citrate Tube beyond the level as illustrated on the reverse side of this guide. Allow the tube to draw the blood from the syringe using a BD Vacutainer® Blood Transfer Device if available. Do not force blood into tube.
• Immediately after draw, gently invert tube 3 to 4 times. Do not shake.
Cat# 363083 363080
Size 13 x 75 mm 13 x 75 mm
Draw 2.7 mL 1.8 mL
Citrate 3.2% (0.109M) 3.2% (0.109M)
BD Global Technical Services: 1.800.631.0174 www.bd.com/vacutainer
BD, BD Logo and BD Vacutainer® are trademarks of Becton, Dickinson and Company. © 2007 BD Patents Pending 10/07 VS5944-3
BD Diagnostics Preanalytical Systems 1 Becton Drive Franklin Lakes, NJ 07417